his tag elisa kit  (Cell Biolabs Inc)

Journal: Bioactive Materials

Article Title: Microenvironment-responsive living hydrogel containing engineered probiotic for treatment of massive bone defects

doi: 10.1016/j.bioactmat.2025.04.020

Figure Lengend Snippet: Construction and performance assessment of the NO-responsive engineered EcN strains. ( A ) Schematic diagram of the engineered strain containing the artificial NO-inducible gene circuits for NO-induced expression of GFP, which serves as a reporter protein, put under the control of the NO-inducible PnorVβ promoter. ( B ) Determination of NO in solutions released by different concentrations of DETA-NONOate. DETA-NONOate gradients used are 0, 50, 100, 200, 400, 600, 800, and 1000 μM. ( C ) The GFP expression levels in the NO sensor (EcN_GFP) strains at various NO donor concentrations. ( D ) GFP fluorescence of EcN_GFP strains with and without the addition of NO donor recorded by confocal laser scanning microscopy (CLSM). In the absence of NO donor, green fluorescence was not observed in the bacteria. ( E ) Induction kinetics of the NorR-based regulatory circuits in EcN under 1000 μM NO donor. ( F ) Schematic diagram of the modified construct for secreted expression of BMP2 after sensing NO stimulation. ( G ) Detection of the BMP2 protein in the supernatant by Western blot. ( H ) BMP2 secreted by EcN strains after 24 h of incubation was quantified using ELISA. If not otherwise specified, the NO donor was used at a concentration of 1000 μM. (All data points represent mean ± standard deviation of three independent replicates. Statistics were derived using the one-way analysis of variance. ∗∗∗P < 0.001.)

Article Snippet: BMP2 concentration was measured using a His-tag ELISA Detection Kit (GenScript, Nanjing, China) according to the manufacturer's protocol.

Techniques: Expressing, Control, Fluorescence, Confocal Laser Scanning Microscopy, Bacteria, Modification, Construct, Western Blot, Incubation, Enzyme-linked Immunosorbent Assay, Concentration Assay, Standard Deviation, Derivative Assay

Journal: Frontiers in Microbiology

Article Title: An optimised promoter and signal peptide improves methionine production of a genetically engineered Candida utilis harboring the δ-zein gene

doi: 10.3389/fmicb.2025.1586229

Figure Lengend Snippet: The effects of different signal peptides on δ-zein secretion were detected by ELISA. **: p ≤ 0.01 (One-Way ANOVA).

Article Snippet: The His-Tag ELISA assay kit was used in accordance with the product’s instruction manual (GenScript, Piscataway, USA).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Immunology

Article Title: Development of a multi-neoepitope vaccine targeting non-small cell lung cancer through reverse vaccinology and bioinformatics approaches

doi: 10.3389/fimmu.2025.1521700

Figure Lengend Snippet: Anti-tumor effects of immunization with the designed MNEV in different study groups. (A) Overview of mouse immunization strategy and analysis schedule. (B) Serum levels of total IgG were determined by ELISA two weeks after the third injection across different groups. Notably, MNEV elicited a significant IgG response following the third injection. (C) The levels of IFN-γ secretion from splenocyte cultures after restimulation with MNEV were assessed using ELISA. Data are presented as means ± standard deviation (SD) for three mice per group. Statistical significance is denoted as follows: ns, not significant; ***P < 0.001; ****P < 0.0001. Mouse immunization workflow, illustrated by BioRender.

Article Snippet: To confirm protein expression and secretion into the conditioned media (CM), a His-tag ELISA kit (GenScript, L00436) was employed, adhering to the manufacturer’s guidelines.

Techniques: Enzyme-linked Immunosorbent Assay, Injection, Standard Deviation

Journal: Frontiers in Immunology

Article Title: Development of a multi-neoepitope vaccine targeting non-small cell lung cancer through reverse vaccinology and bioinformatics approaches

doi: 10.3389/fimmu.2025.1521700

Figure Lengend Snippet: MNEV induction of CD19+ B cells and Granzyme B levels in vitro . (A–C) Flow cytometry plots of CD19+ B cells in splenocytes post-restimulation. (D) Histogram showing the percentages of CD19+ B cells following restimulation assays in splenocytes from study groups. (E) The levels of granzyme B secreted by splenocytes isolated from MNEV-immunized mice two weeks following the final immunization. The supernatants were collected from splenocytes that had been restimulated with MNEV. Granzyme B levels in splenocyte cultures were quantified using the ELISA technique. The data, representative of three independent experiments, are presented as mean values with standard deviations (SD). Notably, MNEV elicited significantly elevated Granzyme B levels compared to control groups, with statistical significance indicated by ns, not significant; ***p < 0.001 and ****P < 0.0001.

Article Snippet: To confirm protein expression and secretion into the conditioned media (CM), a His-tag ELISA kit (GenScript, L00436) was employed, adhering to the manufacturer’s guidelines.

Techniques: In Vitro, Flow Cytometry, Isolation, Enzyme-linked Immunosorbent Assay, Control